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FDX1 Recombinant antibody, PBS Only

FDX1 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230196E9

Cat no : 82957-2-PBS

Synonyms

Adrenal ferredoxin, Adrenodoxin, mitochondrial, ADX, FDX, FDX1, ferredoxin 1, FLJ16057, FLJ32254, Hepatoredoxin, LOH11CR1D, TMEM79, transmembrane protein 79

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FDX1 Recombinant antibody (82957-2-RR)

    WB analysis using 82957-2-RR (same clone as 82957-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82957-2-RR (FDX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FDX1 Recombinant antibody (82957-2-RR)

    IF Staining of HepG2 using 82957-2-RR (same clone as 82957-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (82957-2-RR, Clone: 230196E9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82957-2-PBS targets FDX1 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FDX1 fusion protein Ag3301
Full Name ferredoxin 1
Calculated Molecular Weight 184 aa, 19 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC017063
Gene Symbol FDX1
Gene ID (NCBI) 2230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.