TMEM74 Monoclonal antibody, PBS Only

TMEM74 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H1B7

Cat no : 67773-1-PBS

Synonyms

FLJ30668, TMEM74, transmembrane protein 74

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Validation Data Gallery

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  WB analysis of LNCaP using 67773-1-Ig (same clone as 67773-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67773-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 67773-1-Ig (same clone as 67773-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67773-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67773-1-Ig (same clone as 67773-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67773-1-PBS in a different storage buffer formulation.

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Product Information

67773-1-PBS targets TMEM74 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMEM74 fusion protein Ag22359
• Full Name: transmembrane protein 74
• Calculated Molecular Weight: 305 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC030710
• Gene Symbol: TMEM74
• Gene ID (NCBI): 157753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been rreported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)