TMEM74 Monoclonal antibody
TMEM74 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2H1B7
Cat no : 67773-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, L02 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67773-1-Ig targets TMEM74 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TMEM74 fusion protein Ag22359 |
| Full Name | transmembrane protein 74 |
| Calculated Molecular Weight | 305 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC030710 |
| Gene Symbol | TMEM74 |
| Gene ID (NCBI) | 157753 |
| RRID | AB_2918538 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been rreported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM74 antibody 67773-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
