TMEM59 Recombinant antibody, PBS Only

TMEM59 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240457A1

Cat no : 83656-3-PBS

Synonyms

UNQ169/PRO195, Liver membrane-bound protein, HSPC001, C1orf8, 240457A1

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Validation Data Gallery

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  IF Staining of HepG2 using 83656-3-RR (same clone as 83656-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMEM59 antibody (83656-3-RR, Clone: 240457A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83656-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83656-3-RR (same clone as 83656-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM59 Recombinant antibody (83656-3-RR, Clone:240457A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83656-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83656-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83656-3-RR against Human TMEM59 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83656-3-PBS is TMEM59 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM59 fusion protein Ag1968
• Full Name: transmembrane protein 59
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC016374
• Gene Symbol: TMEM59
• Gene ID (NCBI): 9528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.