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TMEM59 Recombinant antibody

TMEM59 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240457A1

Cat no : 83656-3-RR

Synonyms

UNQ169/PRO195, Liver membrane-bound protein, HSPC001, C1orf8, 240457A1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM59 Recombinant antibody (83656-3-RR)

    IF Staining of HepG2 using 83656-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMEM59 antibody (83656-3-RR, Clone: 240457A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using TMEM59 Recombinant antibody (83656-3-RR)

    FC experiment of HepG2 using 83656-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM59 Recombinant antibody (83656-3-RR, Clone:240457A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83656-3-RR

    Affinity and Kinetic Characterization of 83656-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83656-3-RR against Human TMEM59 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83656-3-RR is TMEM59 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM59 fusion protein Ag1968
Full Name transmembrane protein 59
Calculated Molecular Weight 36 kDa
GenBank Accession NumberBC016374
Gene Symbol TMEM59
Gene ID (NCBI) 9528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.

Protocols

Product Specific Protocols
IF protocol for TMEM59 antibody 83656-3-RRDownload protocol
FC protocol for TMEM59 antibody 83656-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols