TMEM176A Polyclonal antibody, PBS Only

TMEM176A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20378-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of fetal human brain using 20378-1-AP (same clone as 20378-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20378-1-AP (TMEM176A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20378-1-AP (same clone as 20378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20378-1-AP (same clone as 20378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20378-1-AP (same clone as 20378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20378-1-AP (same clone as 20378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20378-1-AP (same clone as 20378-1-PBS)

  Immunofluorescent analysis of Hela cells, using TMEM176A antibody 20378-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

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Product Information

20378-1-PBS targets TMEM176A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM176A fusion protein Ag14194
• Full Name: transmembrane protein 176A
• Calculated Molecular Weight: 235 aa, 26 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC008303
• Gene Symbol: TMEM176A
• Gene ID (NCBI): 55365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.