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TMEM176A Polyclonal antibody

TMEM176A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20378-1-AP

Synonyms

Transmembrane protein 176A, Hepatocellular carcinoma-associated antigen 112, HCA112, GS188

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    WB analysis of fetal human brain using 20378-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20378-1-AP (TMEM176A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of mouse brain using 20378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of mouse brain using 20378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of human liver using 20378-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of human liver using 20378-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM176A Polyclonal antibody (20378-1-AP)

    IF Staining of HeLa using 20378-1-AP

    Immunofluorescent analysis of Hela cells, using TMEM176A antibody 20378-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inmouse brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20378-1-AP targets TMEM176A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM176A fusion protein Ag14194
Full Name transmembrane protein 176A
Calculated Molecular Weight 235 aa, 26 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC008303
Gene Symbol TMEM176A
Gene ID (NCBI) 55365
RRIDAB_10697661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TMEM176A antibody 20378-1-APDownload protocol
IHC protocol for TMEM176A antibody 20378-1-APDownload protocol
IF protocol for TMEM176A antibody 20378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols