TMEM173/STING Recombinant antibody, PBS Only

TMEM173/STING Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7K7

Cat no : 80165-1-PBS

Synonyms

STING, NET23, MPYS, MITA, ERIS

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Validation Data Gallery

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  WB analysis using 80165-1-RR (same clone as 80165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80165-1-RR (TMEM173/STING antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80165-1-RR (same clone as 80165-1-PBS)

  IP result of anti-TMEM173/STING (IP: 80165-1-RR, 4ug; Detection: 80165-1-RR 1:8000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (80165-1-RR, Clone: 7K7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 80165-1-RR (same clone as 80165-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TMEM173/STING antibody (80165-1-RR, Clone: 7K7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80165-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80165-1-RR against Human TMEM173/STING were performed. The affinity constant is 0.776 nM.

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Product Information

80165-1-PBS targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM173/STING fusion protein Ag13921
• Full Name: transmembrane protein 173
• Calculated Molecular Weight: 379 aa, 42 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC047779
• Gene Symbol: STING
• Gene ID (NCBI): 340061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.