TMEM165 Recombinant antibody

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  IF Staining of HepG2 using 83146-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (83146-1-RR, Clone: 230517B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 83146-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM165 Recombinant antibody (83146-1-RR, Clone:230517B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83146-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83146-1-RR against Human TMEM165 were performed. The affinity constant is below 1 pM.

TMEM165 {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83146-1-RR

Clone No.230517B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Transmembrane protein PT27, Transmembrane protein 165, TPARL, TMPT27, Putative divalent cation/proton antiporter TMEM165

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83146-1-RR targets TMEM165 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM165 fusion protein Ag14362
• Full Name: transmembrane protein 165
• Calculated Molecular Weight: 324 aa, 35 kDa
• GenBank Accession Number: BC003545
• Gene Symbol: TMEM165
• Gene ID (NCBI): 55858
• RRID: AB_3670844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HC07
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.