TMEM165 Recombinant antibody, PBS Only (Detector)

TMEM165 Recombinant Antibody for IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230517B1

Cat no : 83146-1-PBS

Synonyms

TMEM165, TMPT27, TPARL, transmembrane protein 165, Transmembrane protein PT27, Transmembrane protein TPARL

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Validation Data Gallery

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  IF Staining of HepG2 using 83146-1-RR (same clone as 83146-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (83146-1-RR, Clone: 230517B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83146-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00217-2

  Cytometric bead array standard curve ofMP00217-2, TMEM165 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83146-2-PBS. Detection antibody: 83146-1-PBS. Standard: Ag14362. Range: 0.625-20 ng/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83146-1-PBS targets TMEM165 as part of a matched antibody pair:

MP00217-2: 83146-2-PBS capture and 83146-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM165 fusion protein Ag14362
• Full Name: transmembrane protein 165
• Calculated Molecular Weight: 324 aa, 35 kDa
• GenBank Accession Number: BC003545
• Gene Symbol: TMEM165
• Gene ID (NCBI): 55858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.