TMEM165 Recombinant antibody, PBS Only (Detector)

TMEM165 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230517B1

Cat no : 83146-1-PBS

Synonyms

Transmembrane protein PT27, Transmembrane protein 165, TPARL, TMPT27, Putative divalent cation/proton antiporter TMEM165

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Validation Data Gallery

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  IF Staining of HepG2 using 83146-1-RR (same clone as 83146-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (83146-1-RR, Clone: 230517B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83146-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83146-1-RR (same clone as 83146-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM165 Recombinant antibody (83146-1-RR, Clone:230517B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83146-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00217-2

  Cytometric bead array standard curve of MP00217-2, TMEM165 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83146-2-PBS. Detection antibody: 83146-1-PBS. Standard: Ag14362. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83146-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83146-1-RR against Human TMEM165 were performed. The affinity constant is below 1 pM.

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Product Information

83146-1-PBS targets TMEM165 as part of a matched antibody pair:

MP00217-2: 83146-2-PBS capture and 83146-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM165 fusion protein Ag14362
• Full Name: transmembrane protein 165
• Calculated Molecular Weight: 324 aa, 35 kDa
• GenBank Accession Number: BC003545
• Gene Symbol: TMEM165
• Gene ID (NCBI): 55858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.