CoraLite® Plus 488-conjugated TLR8 Monoclonal antibody

TLR8 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6A4

Cat no : CL488-67317

Synonyms

CD288, TLR8, toll like receptor 8

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-67317

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 TLR8 antibody (CL488-67317, Clone: 3B6A4 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL488-67317

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 TLR8 antibody (CL488-67317, Clone: 3B6A4 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67317 targets TLR8 in IF-P applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLR8 fusion protein Ag16566
• Full Name: toll-like receptor 8
• Calculated Molecular Weight: 1041 aa, 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC101075
• Gene Symbol: TLR8
• Gene ID (NCBI): 51311
• RRID: AB_2919451
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.