Anti-Human TLR3/CD283 Rabbit Recombinant Antibody

TLR3/CD283 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230445F1

Cat no : 98016-1-RR

Synonyms

TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445F1

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Validation Data Gallery

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  FC experiment of human monocyte-derived immature dendritic cells using 98016-1-RR

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR, Clone: 230445F1) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC anti-human CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human monocyte-derived immature dendritic cells using 98016-1-RR

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR, Clone: 230445F1) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98016-1-RR against Human TLR3/CD283 were performed. The affinity constant is 5.56 nM.

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Tested Applications

• Positive FC (Intra) detected in: human monocyte-derived immature dendritic cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98016-1-RR is TLR3/CD283 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TLR3/CD283 fusion protein Ag34044
• Full Name: toll-like receptor 3
• Calculated Molecular Weight: 904 aa, 104 kDa
• GenBank Accession Number: BC094737
• Gene Symbol: TLR3
• Gene ID (NCBI): 7098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).