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TLR2 Monoclonal antibody
TLR2 Monoclonal Antibody for WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1C1D3
Cat no : 66645-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 1 publications below |
Product Information
66645-1-Ig targets TLR2 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TLR2 fusion protein Ag25626 |
| Full Name | toll-like receptor 2 |
| Calculated molecular weight | 784 aa, 90 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC033756 |
| Gene symbol | TLR2 |
| Gene ID (NCBI) | 7097 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TLR2 antibody 66645-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Transl Med Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease. | ||
Cells Clostridium Butyricum ZJU-F1 Benefits the Intestinal Barrier Function and Immune Response Associated with Its Modulation of Gut Microbiota in Weaned Piglets. | ||
Front Microbiol PPARγ Ameliorates Mycobacterium tuberculosis H37Ra-Induced Foamy Macrophage Formation via the ABCG1-Dependent Cholesterol Efflux Pathway in THP-1 Macrophages. | ||
ACS Omega Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Suppressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal Pathway. | ||
Anim Nutr Lactiplantibacillus plantarum L47 and inulin alleviate enterotoxigenic Escherichia coli induced ileal inflammation in piglets by upregulating the levels of α-linolenic acid and 12,13-epoxyoctadecenoic acid | ||
Front Pharmacol Zhuifeng tougu capsules inhibit the TLR4/MyD88/NF-κB signaling pathway and alleviate knee osteoarthritis: In vitro and in vivo experiments |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (09-27-2019) | Used in an antibody array to identify proteins in human EDTA plasma.
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