TLR2 Monoclonal antibody, PBS Only (Capture)

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  WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  WB result of TLR2 antibody (66645-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR2 transfected THP-1 cells. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66645-1-Ig (same clone as 66645-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66645-1-Ig (TLR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR2 antibody (66645-1-Ig, Clone: 1C1D3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50388-1

  Cytometric bead array standard curve of MP50388-1, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-2-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50388-3

  Cytometric bead array standard curve of MP50388-3, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-4-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

• Featured Product
• KD/KO Validated

TLR2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66645-1-PBS

Clone No.1C1D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2

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Product Information

66645-1-PBS targets TLR2 as part of a matched antibody pair:

MP50388-1: 66645-1-PBS capture and 66645-2-PBS detection (validated in Cytometric bead array)

MP50388-3: 66645-1-PBS capture and 66645-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25626

  Product name: Recombinant human TLR2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 19-302 aa of BC033756

  Sequence: KEESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDR

• Full Name: toll-like receptor 2
• Calculated Molecular Weight: 784 aa, 90 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC033756
• Gene Symbol: TLR2
• Gene ID (NCBI): 7097
• RRID: AB_2882003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60603
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).