TLE3 Recombinant antibody, PBS Only

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  IF Staining of HepG2 using 83955-4-RR (same clone as 83955-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (83955-4-RR, Clone: 240680G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83955-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83955-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83955-4-RR against Human TLE3 were performed. The affinity constant is 0.134 nM.

TLE3 {ptg:Brand} Recombinant Antibody for IF/ICC, ELISA

Cat No. 83955-4-PBS

Clone No.240680G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

GRG3, GRG 3, ESG3, ESG, Enhancer of split groucho-like protein 3

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Product Information

83955-4-PBS targets TLE3 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TLE3 fusion protein Ag1930
• Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Calculated Molecular Weight: 772 aa, 83 kDa
• GenBank Accession Number: BC015729
• Gene Symbol: TLE3
• Gene ID (NCBI): 7090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q04726
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane