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TLE3 Recombinant antibody

TLE3 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240680G3

Cat no : 83955-4-RR

Synonyms

GRG3, GRG 3, ESG3, ESG, Enhancer of split groucho-like protein 3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TLE3 Recombinant antibody (83955-4-RR)

    IF Staining of HepG2 using 83955-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (83955-4-RR, Clone: 240680G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83955-4-RR

    Affinity and Kinetic Characterization of 83955-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83955-4-RR against Human TLE3 were performed. The affinity constant is 0.134 nM.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83955-4-RR is TLE3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TLE3 fusion protein Ag1930
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated Molecular Weight 772 aa, 83 kDa
GenBank Accession NumberBC015729
Gene Symbol TLE3
Gene ID (NCBI) 7090
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Protocols

Product Specific Protocols
IF protocol for TLE3 antibody 83955-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols