CoraLite® Plus 488-conjugated TLE1 Monoclonal antibody

• Featured Product
• KD/KO Validated

TLE1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2H11

Cat no : CL488-66216

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-66216

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 TLE1 antibody (CL488-66216, Clone: 2F2H11 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66216 targets TLE1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLE1 fusion protein Ag21327
• Full Name: transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC010100
• Gene Symbol: TLE1
• Gene ID (NCBI): 7088
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).