PE Anti-Mouse TIGIT (1G9)

TIGIT Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

1G9

Cat no : PE-65079

Synonyms

ENSMUSG00000071552, Tigit, Vstm3

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Validation Data Gallery

• 

  FC experiment of BALB/c mouse splenocytes using PE-65079

  1X10^6 Con A-stimulated (3 ug/ml, 2.5 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 0.5 ug PE Anti-Mouse TIGIT (PE-65079, Clone: 1G9). Cells were not fixed.

• 

  FC experiment of BALB/c mouse splenocytes using PE-65079

  1X10^6 Con A-stimulated (3 ug/ml, 2.5 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 0.5 ug PE-Mouse IgG1 isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65079 targets TIGIT in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: T cell immunoreceptor with Ig and ITIM domains
• GenBank Accession Number: NM_001146325
• Gene Symbol: Tigit
• Gene ID (NCBI): 100043314
• RRID: AB_2883875
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).