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Anti-Mouse TIGIT Rabbit Recombinant Antibody, PBS Only

TIGIT Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240242G1

Cat no : 98026-1-PBS

Synonyms

Vstm3, 240242G1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse TIGIT Rabbit Recombinant Antibody (98026-1-RR)

    FC experiment of mouse splenocytes using 98026-1-RR (same clone as 98026-1-PBS)

    1x10^6 untreated or CD3/CD28 treated mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug Anti-Mouse TIGIT Rabbit Recombinant Antibody (98026-1-RR, Clone:240242G1) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were not fixed. This data was developed using the same antibody clone with 98026-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98026-1-RR

    Affinity and Kinetic Characterization of 98026-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98026-1-RR against Mouse TIGIT were performed. The affinity constant is 1.08 nM.

Product Information

The immunogen of 98026-1-PBS is TIGIT Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name T cell immunoreceptor with Ig and ITIM domains
Calculated Molecular Weight26kDa
GenBank Accession NumberNP_001139797.1
Gene Symbol Tigit
Gene ID (NCBI) 100043314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).