TIAR Monoclonal antibody, PBS Only

TIAR Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E8D12

Cat no : 66907-1-PBS

Synonyms

Nucleolysin TIAR, TCBP, TIA 1 related protein, TIAL1, TIAR

---

Validation Data Gallery

• 

  WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66907-1-Ig (same clone as 66907-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66907-1-Ig (same clone as 66907-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66907-1-Ig (TIAR antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human TIAR (66907-1-Ig, Clone:1E8D12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66907-1-PBS targets TIAR in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TIAR fusion protein Ag11981
• Full Name: TIA1 cytotoxic granule-associated RNA binding protein-like 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 39 and 41 kDa
• GenBank Accession Number: BC030025
• Gene Symbol: TIAL1
• Gene ID (NCBI): 7073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites. 66907-1-Ig detects 39 and 41 kDa bands in SDS-PAGE. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)