TIA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TIA1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

63

Reviews (4)

Cat no : 12133-2-AP

Synonyms

RNA-binding protein TIA-1, RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40

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Validation Data Gallery

• 

  WB analysis of COLO 320 using 12133-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12133-2-AP (TIA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12133-2-AP

  WB result of TIA1 antibody (12133-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIA1 transfected Jurkat cells.

• 

  WB analysis using 12133-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12133-2-AP (TIA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12133-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12133-2-AP (TIA1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12133-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12133-2-AP (TIA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12133-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12133-2-AP (TIA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12133-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12133-2-AP (TIA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12133-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12133-2-AP (TIA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12133-2-AP

  IP result of anti-TIA1 (IP:12133-2-AP, 4ug; Detection:12133-2-AP 1:500) with Jurkat cells lysate 3200ug.

• 

  IHC staining of human lymphoma using 12133-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12133-2-AP (TIA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12133-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12133-2-AP (TIA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12133-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12133-2-AP (TIA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12133-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12133-2-AP (TIA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 12133-2-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using TIA1 antibody (12133-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), 594-CL594-Phalloidin (red).

• 

  IF Staining of L02 using 12133-2-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using TIA1 antibody (12133-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12133-2-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (12133-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12133-2-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (12133-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 12133-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIA1 antibody (12133-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12133-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12133-2-AP (TIA1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12133-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TIA1 (12133-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: COLO 320 cells, Raji cells, mouse spleen tissue, mouse thymus tissue, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lymphoma tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells, HepG2 cells, HeLa cells, sodium arsenite treated HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 38 publications below
• IHC: See 4 publications below
• IF: See 34 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

12133-2-AP targets TIA1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIA1 fusion protein Ag2778
• Full Name: TIA1 cytotoxic granule-associated RNA binding protein
• Calculated Molecular Weight: 214 aa, 24 kDa, 43 kDa
• Observed Molecular Weight: ~40 kDa
• GenBank Accession Number: BC015944
• Gene Symbol: TIA1
• Gene ID (NCBI): 7072
• RRID: AB_2201427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43. Moreover, TDP-43 in SGs became less mobile and insoluble.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun ; KD Validated
human	RIP	Cell Res; Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures.; Authors - Lei Sun
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	IF,IHC	Neuron; TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics.; Authors - Ian R Mackenzie
mouse	WB	Nat Commun; Cellular stress alters 3'UTR landscape through alternative polyadenylation and isoform-specific degradation.; Authors - Dinghai Zheng
mouse	WB	Brain; Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia; Authors - Laura Pasetto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

haibo (Verified Customer) (09-28-2020)

Western blotting was good. But can not see clear granule signal even after treatment by IF. The cells used were fibroblasts.

• Applications: Western Blot, Immunofluorescence

FH

Manohar (Verified Customer) (09-23-2020)

Used 1% Milk for blocking

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

FH

Joshua (Verified Customer) (12-28-2019)

PANC1 cells fixed in 4% paraformaldehyde and stained overnight at 4C. Bright staining, mix of nuclear and stress granule localization as expected.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human pancreatic adenocarcinoma

TIA1 Antibody Immunofluorescence, validation (1:500 dilution) in Human pancreatic adenocarcinoma (Cat no:12133-2-AP)

FH

Joshua (Verified Customer) (12-12-2018)

We found acetone fixation (20 min in 100% acetone at -20C) worked better than paraformaldehyde.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: IMR90 Human Lung Fibroblasts

TIA1 Antibody Immunofluorescence, validation (1:500 dilution) in IMR90 Human Lung Fibroblasts (Cat no:12133-2-AP)

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