THRA Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

THRA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11C8

Cat no : 66703-1-PBS

Synonyms

AR7, c ERBA 1, c erbA alpha, EAR 7, EAR7, ERB T 1, ERBA, ERBA1, NR1A1, THRA, THRA1, THRA2, Thyroid hormone receptor alpha, V erbA related protein 7

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Validation Data Gallery

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  WB analysis of A431 using 66703-1-Ig (same clone as 66703-1-PBS)

  WB result of THRA antibody (66703-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THRA transfected A431 cells. This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66703-1-PBS targets THRA in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: THRA fusion protein Ag27189
• Full Name: thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
• Calculated Molecular Weight: 54 aa, 7 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC002728
• Gene Symbol: THRA
• Gene ID (NCBI): 7067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.