WB result of THRA antibody (66703-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THRA transfected A431 cells.
IHC staining of human thyroid cancer using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, human thyroid cancer tissue, mouse heart tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunohistochemistry (IHC)
IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC
IF/ICC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
WB result of THRA antibody (66703-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THRA transfected A431 cells.
IHC Figures
IHC staining of human thyroid cancer using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 66703-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A431 using 66703-1-Ig
Immunofluorescent analysis of (4% PFA) fixed A431 cells using THRA antibody (66703-1-Ig, Clone: 1A11C8 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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