THOC5 Recombinant antibody

THOC5 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240013F7

Cat no : 83435-1-RR

Synonyms

KIAA0983, Functional spliceosome-associated protein 79, C22orf19, 240013F7

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Validation Data Gallery

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  IF Staining of U2OS using 83435-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 83435-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of U2OS using 83435-1-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thoc5 Recombinant Antibody (83435-1-RR, Clone:240013F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83435-1-RR is THOC5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: THOC5 fusion protein Ag6664
• Full Name: THO complex 5
• Calculated Molecular Weight: 79 kDa
• GenBank Accession Number: BC003615
• Gene Symbol: THOC5
• Gene ID (NCBI): 8563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.