Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of U2OS using 83435-1-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thoc5 Recombinant Antibody (83435-1-RR, Clone:240013F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thoc5 Recombinant Antibody (83435-1-RR, Clone:240013F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of U2OS using 83435-1-RR
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of U2OS using 83435-1-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thoc5 Recombinant Antibody (83435-1-RR, Clone:240013F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
THOC5 Recombinant antibody
Catalog Number
83435-1-RR
Citations
-
Dilutions
IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
