CoraLite® Plus 488-conjugated TGN46 Polyclonal antibody

TGN46 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

Cat no : CL488-13573

Synonyms

TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

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Validation Data Gallery

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  IF Staining of HeLa using CL488-13573

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TGN46 antibody (CL488-13573) at dilution of 1:800.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-13573 targets TGN46 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGN46 fusion protein Ag4470
• Full Name: trans-golgi network protein 2
• Calculated Molecular Weight: 447 aa, 47 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC028219
• Gene Symbol: TGN46
• Gene ID (NCBI): 10618
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Publications

Species	Application	Title
mouse	IF	Elife; TMC7 deficiency causes acrosome biogenesis defects and male infertility in mice; Authors - Jing Wang