TGM2 Monoclonal antibody, PBS Only

TGM2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4C11

Cat no : 68006-1-PBS

Synonyms

TGase C, TGase 2, TG2, TG(C), Erythrocyte transglutaminase

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Validation Data Gallery

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  WB analysis of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 68006-1-Ig (same clone as 68006-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 68006-1-Ig (same clone as 68006-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68006-1-Ig (same clone as 68006-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 68006-1-Ig (same clone as 68006-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 68006-1-Ig (same clone as 68006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68006-1-Ig (TGM2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 68006-1-Ig (same clone as 68006-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TGM2 antibody (68006-1-Ig, Clone: 2D4C11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TGM2 (68006-1-Ig, Clone:2D4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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Product Information

68006-1-PBS targets TGM2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGM2 fusion protein Ag7462
• Full Name: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC003551
• Gene Symbol: TGM2
• Gene ID (NCBI): 7052
• RRID: AB_2918753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.