TGIF2 Monoclonal antibody, PBS Only

TGIF2 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2D6B6

Cat no : 67576-1-PBS

Synonyms

TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, 5 TG 3 interacting factor 2

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Validation Data Gallery

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  WB analysis using 67576-1-Ig (same clone as 67576-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67576-1-Ig (same clone as 67576-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 67576-1-Ig (same clone as 67576-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67576-1-Ig (same clone as 67576-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGIF2 Monoclonal antibody (67576-1-Ig, Clone:2D6B6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

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Product Information

67576-1-PBS targets TGIF2 in Indirect ELISA, WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGIF2 fusion protein Ag30083
• Full Name: TGFB-induced factor homeobox 2
• Calculated Molecular Weight: 237 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012816
• Gene Symbol: TGIF2
• Gene ID (NCBI): 60436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TGIF2, is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein.