TGFBR2 Monoclonal antibody, PBS Only

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  WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 66636-1-Ig (same clone as 66636-1-PBS)

  WB result of TGFBR2 antibody (66636-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBR2 transfected HEK-293T cells. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66636-1-Ig (same clone as 66636-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66636-1-Ig (same clone as 66636-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66636-1-Ig (same clone as 66636-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66636-1-Ig (same clone as 66636-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TGFBR2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66636-1-PBS

Clone No.2D5H7

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, ELISA

Synonyms

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2

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Product Information

66636-1-PBS targets TGFBR2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGFBR2 fusion protein Ag25773
• Full Name: transforming growth factor, beta receptor II (70/80kDa)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65-80 kDa
• GenBank Accession Number: BC040499
• Gene Symbol: TGFBR2
• Gene ID (NCBI): 7048
• RRID: AB_2881995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P37173
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.