- Featured Product
- KD/KO Validated
TGFBR2 Monoclonal antibody
TGFBR2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat and More (4)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2D5H7
Cat no : 66636-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HEK-293T cells, U2OS cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells, NCCIT cells, L02 cells, K-562 cells, HepG2 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 32 publications below |
IHC | See 5 publications below |
IF | See 5 publications below |
IP | See 2 publications below |
CoIP | See 2 publications below |
Product Information
66636-1-Ig targets TGFBR2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.
Tested Reactivity | Human, Mouse , Rat |
Cited Reactivity | human, mouse, rat, goat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TGFBR2 fusion protein Ag25773 |
Full Name | transforming growth factor, beta receptor II (70/80kDa) |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 65-80 kDa |
GenBank Accession Number | BC040499 |
Gene Symbol | TGFBR2 |
Gene ID (NCBI) | 7048 |
RRID | AB_2881995 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TGFBR2 antibody 66636-1-Ig | Download protocol |
IHC protocol for TGFBR2 antibody 66636-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Med Engineering irradiated tumor-derived microparticles as personalized vaccines to enhance anti-tumor immunity | ||
Acta Pharmacol Sin GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy | ||
Br J Pharmacol Inhibition of HSP90 S-nitrosylation Alleviates Cardiac Fibrosis via TGFβ/SMAD3 Signaling Pathway. | ||
NPJ Regen Med Alkaline shear-thinning micro-nanocomposite hydrogels initiate endogenous TGFβ signaling for in situ bone regeneration | ||
Acta Pharmacol Sin N-n-Butyl haloperidol iodide ameliorates liver fibrosis and hepatic stellate cell activation in mice.
| ||
Aging (Albany NY) A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (08-15-2023) | This antibody worked well for labeling TGF beta receptor type 2 in mouse kidney. We were able to acquire robust immunofluorescence signals with this KO validated antibody.
|