TGFBI / BIGH3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TGFBI / BIGH3 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E11D11

Cat no : 60007-1-PBS

Synonyms

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

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Validation Data Gallery

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  WB analysis of human kidney using 60007-1-Ig (same clone as 60007-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60007-1-Ig (same clone as 60007-1-PBS)

  IP result of anti-TGFBI / BIGH3 (IP:60007-1-Ig, 4ug; Detection:60007-1-Ig 1:300) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TGFBI / BIGH3 antibody (60007-1-Ig, Clone: 3E11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TGFBI / BIGH3 antibody (60007-1-Ig, Clone: 3E11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60007-1-PBS targets TGFBI / BIGH3 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGFBI / BIGH3 fusion protein Ag0241
• Full Name: transforming growth factor, beta-induced, 68kDa
• Calculated Molecular Weight: 683 aa, 75 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC000097
• Gene Symbol: TGFBI
• Gene ID (NCBI): 7045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).