TFPI Monoclonal antibody

TFPI Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B12F11

Cat no : 66842-1-Ig

Synonyms

EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1

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Validation Data Gallery

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  WB analysis of human placenta using 66842-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66842-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66842-1-Ig targets TFPI in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFPI fusion protein Ag14980
• Full Name: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Calculated Molecular Weight: 251aa,29 kDa; 304aa,35 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC015514
• Gene Symbol: TFPI
• Gene ID (NCBI): 7035
• RRID: AB_2882183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.; Authors - Ting Li