TFF2 Monoclonal antibody, PBS Only

TFF2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7B1

Cat no : 66471-1-PBS

Synonyms

SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2

---

Validation Data Gallery

• 

  WB analysis of rat pancreas using 66471-1-Ig (same clone as 66471-1-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig stomach using 66471-1-Ig (same clone as 66471-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig pancreas using 66471-1-Ig (same clone as 66471-1-PBS)

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig stomach using 66471-1-Ig (same clone as 66471-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig pancreas using 66471-1-Ig (same clone as 66471-1-PBS)

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66471-1-PBS in a different storage buffer formulation.

---

Product Information

66471-1-PBS targets TFF2 in WB, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFF2 fusion protein Ag18765
• Full Name: trefoil factor 2
• Calculated Molecular Weight: 129 aa, 14 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC032820
• Gene Symbol: TFF2
• Gene ID (NCBI): 7032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system.