TFF2 Monoclonal antibody

TFF2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2D7B1

Cat no : 66471-1-Ig

Synonyms

SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2

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Validation Data Gallery

• 

  WB analysis of rat pancreas using 66471-1-Ig

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig stomach using 66471-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig pancreas using 66471-1-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat pancreas tissue, pig pancreas tissue, pig stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66471-1-Ig targets TFF2 in WB,ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFF2 fusion protein Ag18765
• Full Name: trefoil factor 2
• Calculated Molecular Weight: 129 aa, 14 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC032820
• Gene Symbol: TFF2
• Gene ID (NCBI): 7032
• RRID: AB_2881837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system.