TFEB Monoclonal antibody, PBS Only (Capture)

TFEB Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2D9

Cat no : 68632-1-PBS

Synonyms

TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB

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Validation Data Gallery

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  WB analysis using 68632-1-Ig (same clone as 68632-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68632-1-Ig (TFEB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68632-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50099-1

  Cytometric bead array in cell lysate using MP50099-1, TFEB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68632-1-PBS. Detection antibody: 68632-2-PBS. Cell lysate: Raji，HepG2，HEK293.

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  Cytometric bead array standard curve of MP50099-1

  Cytometric bead array standard curve of MP50099-1, TFEB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68632-1-PBS. Detection antibody: 68632-2-PBS. Standard:Ag24850. Range: 0.195-50 ng/mL.

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Product Information

68632-1-PBS targets TFEB as part of a matched antibody pair:

MP50099-1: 68632-1-PBS capture and 68632-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFEB fusion protein Ag24850
• Full Name: transcription factor EB
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 53, 65-70 kDa
• GenBank Accession Number: BC032448
• Gene Symbol: TFEB
• Gene ID (NCBI): 7942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).