TEX14 Polyclonal antibody, PBS Only

TEX14 Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18351-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18351-1-AP (TEX14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

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Product Information

18351-1-PBS targets TEX14 in IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX14 fusion protein Ag13177
• Full Name: testis expressed 14
• Calculated Molecular Weight: 957aa,107 kDa; 1497aa,168 kDa
• Observed Molecular Weight: 180-200 kDa, 106 kDa
• GenBank Accession Number: BC040526
• Gene Symbol: TEX14
• Gene ID (NCBI): 56155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Tex14 has several variant isoforms with the MW from about 100 kDa to 168 kDa.