TEX14 Polyclonal antibody

TEX14 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18351-1-AP

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

---

Validation Data Gallery

• 

  WB analysis of mouse testis using 18351-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18351-1-AP (TEX14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

The immunogen of 18351-1-AP is TEX14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX14 fusion protein Ag13177
• Full Name: testis expressed 14
• Calculated Molecular Weight: 957aa,107 kDa; 1497aa,168 kDa
• Observed Molecular Weight: 180-200 kDa, 106 kDa
• GenBank Accession Number: BC040526
• Gene Symbol: TEX14
• Gene ID (NCBI): 56155
• RRID: AB_10641992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Tex14 has several variant isoforms with the MW from about 100 kDa to 168 kDa.

Publications

Species	Application	Title
human	WB,IF	Int J Mol Sci; MCF7 Spheroid Development: New Insight about Spatio/Temporal Arrangements of TNTs, Amyloid Fibrils, Cell Connections, and Cellular Bridges.; Authors - Laura Pulze
human,mouse	IF	FASEB J; Dazl determines primordial follicle formation through the translational regulation of Tex14.; Authors - Roseanne Rosario
mouse	IHC	Methods Mol Biol; Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.; Authors - Kathrin Gassei
mouse	IF	Cell Death Dis; Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis.; Authors - Wen-Long Lei
mouse	IF	Cells; The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication; Authors - Pablo López-Jiménez