TET3 Polyclonal antibody

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  WB analysis using 27150-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27150-1-AP (TET3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TET3 Polyclonal Antibody for WB, ELISA

Cat No. 27150-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80

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Tested Applications

• Positive WB detected in: MCF-7 cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27150-1-AP targets TET3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TET3 fusion protein Ag25877
• Full Name: tet oncogene family member 3
• Calculated Molecular Weight: 1660 aa, 179 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC022243
• Gene Symbol: TET3
• Gene ID (NCBI): 200424
• RRID: AB_3669585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).