TERF2 Monoclonal antibody, PBS Only

TERF2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B1E1

Cat no : 66893-1-PBS

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

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Validation Data Gallery

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  WB analysis of MCF-7 using 66893-1-Ig (same clone as 66893-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66893-1-Ig (same clone as 66893-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 66893-1-Ig (same clone as 66893-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66893-1-Ig (same clone as 66893-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66893-1-Ig (same clone as 66893-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66893-1-Ig (same clone as 66893-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66893-1-Ig (same clone as 66893-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66893-1-Ig (same clone as 66893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66893-1-Ig (same clone as 66893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66893-1-Ig (same clone as 66893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66893-1-Ig (same clone as 66893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

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Product Information

66893-1-PBS targets TERF2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TERF2 fusion protein Ag28513
• Full Name: telomeric repeat binding factor 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60-65.32-35 kDa
• GenBank Accession Number: BC024890
• Gene Symbol: TERF2
• Gene ID (NCBI): 7014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.