Filter: AllWBIHC

WB analysis of MCF-7 using 66893-1-Ig (same clone as 66893-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66893-1-Ig (same clone as 66893-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of 4T1 using 66893-1-Ig (same clone as 66893-1-PBS)
4T1 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of Daudi using 66893-1-Ig (same clone as 66893-1-PBS)
Daudi cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HT-29 using 66893-1-Ig (same clone as 66893-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 66893-1-Ig (same clone as 66893-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 66893-1-Ig (same clone as 66893-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

TERF2 Monoclonal antibody, PBS Only

TERF2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66893-1-PBS

Clone No.5B1E1

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66893-1-PBS targets TERF2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	TERF2 fusion protein Ag28513 Predict reactive species
Full Name	telomeric repeat binding factor 2
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	60-65.32-35 kDa
GenBank Accession Number	BC024890
Gene Symbol	TERF2
Gene ID (NCBI)	7014
RRID	AB_2882222
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q15554
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.