TERF2 Monoclonal antibody

TERF2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

5B1E1

Cat no : 66893-1-Ig

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

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Validation Data Gallery

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  WB analysis of MCF-7 using 66893-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66893-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66893-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66893-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66893-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66893-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66893-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66893-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66893-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66893-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66893-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, HT-29 cells, K-562 cells, HEK-293 cells, Daudi cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human gliomas tissue, rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

66893-1-Ig targets TERF2 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TERF2 fusion protein Ag28513
• Full Name: telomeric repeat binding factor 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60-65.32-35 kDa
• GenBank Accession Number: BC024890
• Gene Symbol: TERF2
• Gene ID (NCBI): 7014
• RRID: AB_2882222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.

Publications

Species	Application	Title
human	IF	Sci Adv; Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).; Authors - Ji Hoon Lee
human,mouse	WB	Front Pharmacol; Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease.; Authors - Xiaoyu Qi
human	WB,ChIP	Nat Commun; Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence; Authors - Athanasios Siametis
mouse	WB	Acta Biochim Biophys Sin (Shanghai); miR-29b-1-5p exacerbates myocardial injury induced by sepsis in a mouse model by targeting TERF2; Authors - Yaqing Jiang