TERF1 Monoclonal antibody

TERF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D10G4

Cat no : 67592-1-Ig

Synonyms

Telomeric repeat-binding factor 1, t TRF1, PIN2, NIMA-interacting protein 2, hTRF1 AS

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Validation Data Gallery

• 

  WB analysis of K-562 using 67592-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67592-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67592-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

67592-1-Ig targets TERF1 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TERF1 fusion protein Ag12540
• Full Name: telomeric repeat binding factor (NIMA-interacting) 1
• Calculated Molecular Weight: 439 aa, 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC029378
• Gene Symbol: TERF1
• Gene ID (NCBI): 7013
• RRID: AB_2918494
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Telomeres are nucleoprotein complexes located at chromosome ends that consist of tandem arrays of TTAGGG repeats bound to specific proteins. Telomeres protect chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. TERF1 (telomeric repeat-binding factor 1) binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. It's one component of the shelterin complex, which involved in the regulation of telomere length and protection.

Publications

Species	Application	Title
human	WB,IP,ChIP	Nat Commun; Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence; Authors - Athanasios Siametis