APC Anti-Mouse TER-119 (TER-119)

TER-119 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

TER-119

Cat no : APC-65149

Synonyms

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

---

Validation Data Gallery

• 

  FC experiment of mouse bone marrow cells using APC-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and 0.50 ug APC Anti-Mouse TER-119 (APC-65149, Clone: TER-119). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using APC-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and 0.50 ug APC-Rat IgG2b isotype control antibody. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using APC-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug APC-Anti-Mouse TER-119 (APC-65149, clone TER-119) (red) or 0.20 ug APC-Rat IgG2b isotype control (blue). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65149 targets TER-119 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Day-14 fetal liver cells from a C57BL/6 mouse
• Full Name: lymphocyte antigen 76
• Gene Symbol: Ly76
• Gene ID (NCBI): 104231
• RRID: AB_2883028
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).