TEM1 Monoclonal antibody, PBS Only

TEM1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9B4

Cat no : 60170-1-PBS

Synonyms

CD164L1, CD248, CD248 molecule, endosialin, Endosialin, TEM1, Tumor endothelial marker 1

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Validation Data Gallery

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  WB analysis using 60170-1-Ig (same clone as 60170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody) at dilution of 1:23000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 60170-1-Ig (same clone as 60170-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60170-1-Ig (same clone as 60170-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:1800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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Product Information

60170-1-PBS targets TEM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TEM1 fusion protein Ag13334
• Full Name: CD248 molecule, endosialin
• Calculated Molecular Weight: 757 aa, 81 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC051340
• Gene Symbol: TEM1
• Gene ID (NCBI): 57124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.