TEM1 Monoclonal antibody

TEM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

1F9B4

Cat no : 60170-1-Ig

Synonyms

CD164L1, CD248, CD248 molecule, endosialin, Endosialin, TEM1, Tumor endothelial marker 1

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Validation Data Gallery

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  WB analysis using 60170-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody) at dilution of 1:23000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 60170-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 60170-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:1800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HUVEC cells, COLO 320 cells, bEnd.3 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

60170-1-Ig targets TEM1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TEM1 fusion protein Ag13334
• Full Name: CD248 molecule, endosialin
• Calculated Molecular Weight: 757 aa, 81 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC051340
• Gene Symbol: TEM1
• Gene ID (NCBI): 57124
• RRID: AB_10694834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.

Publications

Species	Application	Title
mouse	WB,IF,IP	Cancer Res; CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer; Authors - Chia-Lun Hong
human	WB	Proc Natl Acad Sci U S A; A membrane protein display platform for receptor interactome discovery.; Authors - Shengya Cao
human	IHC	Oncotarget; Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma.; Authors - Emily Capone
human,mouse,rat	WB,IHC,IF,IP	Sci Rep; Tumor endothelial marker 1 is upregulated in heart after cardiac injury and participates in cardiac remodeling.; Authors - Po-Sheng Chen
human,rat	WB,IF	Front Cardiovasc Med; Soluble tumor endothelial marker 1 in heart failure with reduced ejection fraction: A pilot study; Authors - Wen-Han Feng
mouse	WB,CoIP	EBioMedicine; CD248 promotes insulin resistance by binding to the insulin receptor and dampening its insulin-induced autophosphorylation; Authors - Patricia O Benedet ; KO Validated