TCL1A Recombinant antibody

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  WB analysis of Ramos using 85129-5-RR

  Ramos cells were subjected to SDS PAGE followed by western blot with 85129-5-RR (TCL1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Ramos using 85129-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using TCL1A antibody (85129-5-RR, Clone: 242623A9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85129-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85129-5-RR against Human TCL1A were performed. The affinity constant is 0.185 nM.

TCL1A {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85129-5-RR

Clone No.242623A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TCL1, T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1, 242623A9

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Tested Applications

• Positive WB detected in: Ramos cells
• Positive IF/ICC detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85129-5-RR targets TCL1A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TCL1A fusion protein Ag0786
• Full Name: T-cell leukemia/lymphoma 1A
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC005831
• Gene Symbol: TCL1A
• Gene ID (NCBI): 8115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P56279
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCL1A, also named as TCL1 and p14 TCL1, enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. TCL1A immunodetection is an independent marker of adverse outcome that could be used in routine settings for the management of DLCL patients. TCL1A expression is correlated with shorter time to treatment in chronic lymphocytic leukemia cases and shorter lymphoma-specific survival in mantle cell lymphoma series. It is a potential therapeutic target.