TBR1 Monoclonal antibody, PBS Only

TBR1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5C3A5

Cat no : 66564-1-PBS

Synonyms

T box brain protein 1, T box, brain, 1, T brain 1, TBR 1, TBR1, TES 56

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Validation Data Gallery

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  WB analysis using 66564-1-Ig (same clone as 66564-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  WB analysis of pig cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66564-1-Ig (TBR1 antibody) at dilution of 1:100 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66564-1-Ig (TBR1 antibody) at dilution of 1:100 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66564-1-PBS targets TBR1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

• Tested Reactivity: Human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBR1 fusion protein Ag15262
• Full Name: T-box, brain, 1
• Calculated Molecular Weight: 682 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC104844
• Gene Symbol: TBR1
• Gene ID (NCBI): 10716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

TBR1, also named as T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.