TBP Monoclonal antibody, PBS Only

TBP Monoclonal Antibody for WB, IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3B2

Cat no : 66166-1-PBS

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

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Validation Data Gallery

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  WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

  Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66166-1-Ig (same clone as 66166-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

  IP result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

  After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66166-1-Ig (same clone as 66166-1-PBS)

  I After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66166-1-Ig (same clone as 66166-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66166-1-Ig (same clone as 66166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (66166-1-Ig, Clone: 2H3B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66166-1-Ig (same clone as 66166-1-PBS)

  1X10^6 HepG2 cells were stained with 0.20ug TBP antibody (66166-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66166-1-PBS targets TBP in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBP fusion protein Ag12383
• Full Name: TATA box binding protein
• Calculated Molecular Weight: 338 aa, 38 kDa
• Observed Molecular Weight: mouse/rat 33-36 kDa and human 37-43kDa
• GenBank Accession Number: BC110341
• Gene Symbol: TBP
• Gene ID (NCBI): 6908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).