TBCD Recombinant antibody, PBS Only

TBCD Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230209H7

Cat no : 82911-1-PBS

Synonyms

SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D

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Validation Data Gallery

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  WB analysis using 82911-1-RR (same clone as 82911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82911-1-RR (TBCD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82911-1-RR (same clone as 82911-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TBCD antibody (82911-1-RR, Clone: 230209H7 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 82911-1-RR (same clone as 82911-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-1-RR, Clone:230209H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82911-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82911-1 -RR against Human TBCD were performed. The affinity constant is 1.52 nM.

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Product Information

82911-1-PBS targets TBCD in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TBCD fusion protein Ag6310
• Full Name: tubulin folding cofactor D
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC003094
• Gene Symbol: TBCD
• Gene ID (NCBI): 6904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.