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TBCD Recombinant antibody

TBCD Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230209H7

Cat no : 82911-1-RR

Synonyms

SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBCD Recombinant antibody (82911-1-RR)

    WB analysis using 82911-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82911-1-RR (TBCD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TBCD Recombinant antibody (82911-1-RR)

    IF Staining of MCF-7 using 82911-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TBCD antibody (82911-1-RR, Clone: 230209H7 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • Flow cytometry (FC) experiment of MCF-7 cells using TBCD Recombinant antibody (82911-1-RR)

    FC experiment of MCF-7 using 82911-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-1-RR, Clone:230209H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82911-1-RR

    Affinity and Kinetic Characterization of 82911-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82911-1 -RR against Human TBCD were performed. The affinity constant is 1.52 nM.

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82911-1-RR targets TBCD in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TBCD fusion protein Ag6310
Full Name tubulin folding cofactor D
Calculated Molecular Weight 133 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC003094
Gene Symbol TBCD
Gene ID (NCBI) 6904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TBCD antibody 82911-1-RRDownload protocol
IF protocol for TBCD antibody 82911-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols