TBC1D4 Polyclonal antibody, PBS Only

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24667-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 24667-1-AP (same clone as 24667-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 24667-1-AP (same clone as 24667-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24667-1-AP (same clone as 24667-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

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Product Information

24667-1-PBS targets TBC1D4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBC1D4 fusion protein Ag20327
• Full Name: TBC1 domain family, member 4
• Calculated Molecular Weight: 1299 aa, 147 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC151239
• Gene Symbol: TBC1D4
• Gene ID (NCBI): 9882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.