TBC1D4 Polyclonal antibody

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24667-1-AP

Synonyms

TBC1 domain family, member 4, TBC1 domain family member 4, KIAA0603, AS160, Akt substrate of 160 kDa

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Validation Data Gallery

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  WB analysis using 24667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 24667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 24667-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24667-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 24667-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse colon using 24667-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 24667-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 24667-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 24667-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells
• Positive IHC detected in: mouse colon tissue, human colon tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24667-1-AP targets TBC1D4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBC1D4 fusion protein Ag20327
• Full Name: TBC1 domain family, member 4
• Calculated Molecular Weight: 1299 aa, 147 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC151239
• Gene Symbol: TBC1D4
• Gene ID (NCBI): 9882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.